Taq DNA Polymerase

Magigen MagicScript Taq DNA Polymerase | Taq Polymerase

● Product Name: Magigen Salt Hotstart Taq DNA Polymerase 2.0 with probe buffer 5000U

● Catalog#: A012S

● Size: 250U

● Taq Polymerase Price: 59USD

● Contents

*1. This enzyme is more suitable for high salt environments, with a unit salt ion concentration up to 160mM.

*2.   The concentrations of K⁺, NH⁴⁺, and Mg²⁺ are 47.5mM, 55mM, and 4 mM, respectively.

● Advantages and Features

Magigen Hotstart Taq DNA Polymerase 2.0 is an antibody-mediated thermostable DNA polymerase, where Taq is point mutated thermostable DNA polymerase. Compared to the wild-type Taq Polymerase, this product has better salt tolerance³. At the same time, the half-life increases to 30 minutes at 95 ℃, and the reaction rate reaches 15s/kb. After heating for 30 seconds, it can be fully activated, exhibiting 5 '-3' DNA polymerase activity and double stranded DNA specific 5 '-3' exonuclease activity. No 3 '-5' exonuclease activity. PCR amplification products using this product has a base "A" attached to the 3 'end, which can be directly used for TA cloning.

Magigen Taq Polymerase has a reaction system for probe qPCR with gene specific primers (GSP).

*3. In probe-based qPCR,   when the concentration of monovalent salt in the system is below 75mM, it will make   the fluorescence curve has a decrease plateau period or inability to reach a significant plateau period, but it does not affect the CT value.

● Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C with the activated salmon sperm DNA as the template-primer.

● Storage Conditions and Shelf Life

Sealed and stored at -20 ℃,   12 months.

➢ What is Taq Polymerase used for?

◆for Dye-based PCR reaction

◆for probe-based PCR reaction, recommended to use 2 × Reaction Buffer 2

◆for RT-PCR

● Recommended Magigen Taq Reaction System

Composition                                                   Vol

2×Reaction Buffer 2                                        12.5 μL

Hotstart Taq Polymerase 2.0                           ~0.25μL

Foward Primer(10 μM)                                    0.5 μL

Reverse Primer(10 μM)                                   0.5 μL

template                                                        1 pg~100 ng

Probe(10μM)⁴                                                 0.25 μL

ddH₂O                                                            Up to 25 μL

*4. Added When performing fluorescence qPCR.

● Recommended amplification procedure

Cycle step                      Temperature       Time          Number of cycles

Pre denaturation           95 ° C            30s-4min⁵         1

Denaturation                 95 ℃             15sec                45

Annealing/Extension     60 ℃            30sec   Fluorescence collected  

*5. The denaturation time can be increased to 4min for complex template.

● Related Taq Polymerase Price (USD)

● Note:

Magigen Hotstart Taq DNA Polymerase is a qualified enzyme with good price.

The hotstart taq polymerase price will be subject to change without prior notice due to currency fluctuation.

All Pictures shown are for illustration purpose only and shall not form part of any offer, contract.