Nucleic Acid Test

Reverse Transcription Kit

● Product Name: Magigen Reverse Transcriptase II - RT enzyme   For RT-PCR Test

● Catalog# : A001S

● Size: 100µl , 200U/ µl

● Reverse Transcriptase Price: 71USD

● Contents

Composition                                                            Vol

● Magigen RT enzyme Features

Magicscript Reverse Transcriptase, RTase II, is a recombinant DNA polymerase. It is a product of the pol gene of   Moloney Murine Leukemia Virus （M MLV）. Based on the reduction of RNase H activity ,   genetically engineered,   Magicscript Reverse Transcriptase II works perfect over a wider temperature range with higher thermal stability and longer half-life.

● Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(rA)•oligo(dT) as template/primer.

● Storage Conditions and Expiration
Sealed at-20℃, valid for 12 months.

● Product Performances
Comparing Magicscript RT-II with same products from both domestic and oversea suppliers under same reacting conditions of 42℃ and 55℃, the results present reliability and stability, especially the superiority in higher temperature zone.

● Magicscript Reverse Transcriptase II Advantages

1.   A wider thermal resistance range of   37°C to 65°C compared with other M-MLV product, and high activity. The residual activity retains at least 70% after 30 min in the high temperature   range of 55°C to 65°C.

2.   Work with kinds of reaction buffers, compatible with one-step reaction systems such as RT-PCR\RT-RDA\RT-Lamp\RT-HDA.

3. Show a perfect linear relationship between the different RNA concentration and the cDNA yield.

● How to use thermostable Reverse Transcriptase II

Example:   first strand cDNA synthesis

Reaction System                    Vol ( µl)

Thermostable RTase II                1

5× Reaction Buffer 1                  4

100 mM DTT                             2

RNase Inhibitor（20-40 U/µl）   1

dNTP Mix ( 25 mM each)           1

primer                                       10μM-250μM

template                                   1pg-100ng

ddH₂O                                      Up to 20-25

● Reaction steps

1. gently mix the reaction system;

2. recommended reaction procedure:

Temperature                Time

25℃¹                              2min

45℃                        30min

85℃                          2min

*1.   This step is only required when using random hexmers;

● RTase II Kit Application

The obtained cDNA can be directly used for 2nd strand cDNA synthesis or PCR amplification.

● Heat Inactivation

80°C for 20 minutes

● Note

1. The optimal temperature range of magicscript thermostable reverse transcriptase II is 37-45 ℃, Its RNaseH activity was one-third that of wild-type MLV. When the temperature is higher than 45 ℃ (lower than 65 ℃), the residual activity will decrease correspondingly with the increase of temperature, but at least 70% of the activity can still be maintained.

2. When using this product for one-step RT-PCR experiments, it is recommended to add the following components to the complete PCR system (including templates, specific primers and other components) that researchers have:

Reaction System                      Vol ( µl)

Thermostable RTase II                1

RNase inhibitor（20-40 U/µl）   1

Final volume                              20-25

Suggested reaction procedure:

45-50℃                         15 min

PCR procedure

● Notice

Magigen Reverse Transcription Kit is a high quality product with a good price.

The Reverse Transcription Kit price is subject to change without prior notice due to currency fluctuation.

The Reverse Transcription Kit price is FOB price, not include tariffs, freight, insurance and other expenses. Some products need to be delivered by dry ice. Please consult the sales manager for details before purchasing.

All Pictures shown are for illustration purpose only and shall not form part of any offer, contract.

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