Magigen CRISPR SpRYCas9 protein is the most compatible SpCas9 mutant for PAM sequences, it is almost completely free from the restriction of PAM sequences.
The feature of the PAM sequence it recognized is NNN(NRN > NYN).
【Catalog#】
C004S
【Introduction】
With its pure protein system that resists distraction and low off-target rate, The CRISPR-Cas9 technologies is a safe and common method in the field of gene editing.
【Unit Definition】
♦ CRISPR Cas9 nuclease is a recombinant protein that carries NLS fragments at both the N- and C-terminals.
♦ This product 1pmol=154 ng.
♦ The design and stabilization of sgRNAs have a great influence on the reaction system, and whether sgRNAs can be degraded by gel electrophoresis before the reaction is carried out.
【Product Features】
The RNP system of Cas protein does not required clone construction, no need for vectors and viruses. Its features of low toxicity, quick, efficient, low off-target rates, and able to use in multi-target editing allow it to be used in gene therapy for many diseases such as blood diseases and tumors.
【Storage Conditions and Expiration】
Sealed at -20℃
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