Isothermal amplification of nucleic acids

Bsu Polymerase | Bsu DNA polymerase

● Product Name: Magigen Bsu DNA polymerase / Bsu Polymerase, Large Fragment

● Catalog#: D004M

● Size: 2500U

● Bsu Polymerase Price: 410 USD

● Contents

Composition                                               Vol

10×Reaction Buffer 9^*1                             1ml×5

Bsu DNA Polymerase, Large Fragment      500µl（5U/µl）

*1 500 mM NaCl, 100 mM Tris-HCl (Ph7.5), 100mM MgCl2, 10mM DTT。

● Advantages and Features

Magigen Bsu Polymerase, derived from Bacillus subtilis, was obtained by truncating the first 296 AAs of the Bacillus subtilis DNA polymerase I gene. This polymerase retains its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. Bsu DNA Polymerase naturally lacks the 3’→ 5’ exonuclease activity, which can be used for recombinase amplification.

Bsu DNA polymerase is a DNA isothermal amplification polymerase with strand displacement activity, and the optimum temperature is 37℃-40℃.

● Storage Conditions and Shelf Life

Stored at -20 ℃ for 12 months

● Application

1. Random primer labeling;

2. Synthesis of the second strand cDNA;

3. Tailing of single dA;

4. DNA strand displacement synthesis.

● Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C

● Heat Inactivation

75°C for 20 minutes

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