Isothermal amplification of nucleic acids

Bsu Polymerase | Bsu DNA polymerase

● Product Name: Magigen Bsu DNA polymerase / Bsu Polymerase, Large Fragment

● Catalog#: D004S

● Size: 250U

● Bsu Polymerase Price: 61 USD

● Contents

Composition                                               Vol

10×Reaction Buffer 9^*1                             1ml×1

Bsu DNA Polymerase, Large Fragment      50µl（5U/µl）

*1 500 mM NaCl, 100 mM Tris-HCl (Ph7.5), 100mM MgCl2, 10mM DTT。

● Advantages and Features

Magigen Bsu Polymerase, derived from Bacillus subtilis, was obtained by truncating the first 296 AAs of the Bacillus subtilis DNA polymerase I gene. This polymerase retains its 5’→ 3’ DNA polymerase activity with its 5’→ 3’ exonuclease domain removed. Bsu DNA Polymerase naturally lacks the 3’→ 5’ exonuclease activity, which can be used for recombinase amplification.

Bsu DNA polymerase is a DNA isothermal amplification polymerase with strand displacement activity, and the optimum temperature is 37℃-40℃.

● Storage Conditions and Shelf Life

Stored at -20 ℃ for 12 months

● Application

1. Random primer labeling;

2. Synthesis of the second strand cDNA;

3. Tailing of single dA;

4. DNA strand displacement synthesis.

● Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C

● Heat Inactivation

75°C for 20 minutes

Magigen MagicScript Bsu DNA polymerase is a qualified enzyme with good price.

The Bsu polymerase price will be subject to change without prior notice due to currency fluctuation.

All Pictures shown are for illustration purpose only and shall not form part of any offer, contract.

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