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➢ Product Name: Magigen Thermostable RNase HII Kit | Ribonuclease RNase H2 Kit

➢ Catalog#: A006M

➢ RNase HII characteristics and functions

Magigen Thermostable RNase HII | Ribonuclease RNase H2 is an endoribonuclease that specially cleaves 5´ end to a ribonucleotide within the context of a double stranded DNA.

Magigen Thermostable RNase HII leaves 3´ hydroxyl and 5´ phosphate ends. RNase H2 will also cleave at multiple sites along the RNA portion of an Okazaki fragment.

The Magigen thermostable RNase HII enzyme, derived from an artificially engineered thermophilus rnhb gene with a molecular weight of 36 KD and a C-end carrying a histidine tag (his tag), has a wider temperature applicable region as well as more rapid nicking efficiency than E.coli RNase HII and wild-type Tth RNase H2, and can be used for rhPCR reactions.

➢ Contents

➢ Unit Definition

One unit is defined as the amount of enzyme required to yield a fluorescence signal consistent with the nicking of 100 pmol of synthetic double-stranded RNA substrate   containing a single ribonucleotide near the quencher of a fluorophore/quencher pair in 15 minutes at 60°C in 1X ThermoPol Buffer.

➢ Storage Conditions and Shelf Life

Sealed and stored at -20 ℃,   12 months.

➢   How to use RNase HII?

Reagent                                    Test        Control (N)           Vol (μL)

10× Reaction Buffer   6            1×             1×                     2.5

RNaseH probe                     200 nM        200 nM               1

RNase HⅡ                           2.5U             2.5U                    0.5

template Tm 59.5℃             20 nM           --                       0.5

Total Vol（add H₂O）                                                         25

60℃，60min。

RNase HII Test Data

Fig1. Magigen RNase HII Test VS Control N

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