Magigen CRISPR Cas12 protein | AapCas12b Protein Kit
Magigen CRISPR Cas12 protein product AapCas12b is an RNA-guided endonuclease. It can specifically cleave target double-stranded DNA in the presence of PAM on target DNA.
This kit is suitablefor the development of detection products based on CRISPRplatform.
Contents of the kit
10×Cas12b Reaction Buffer
1×Cas12b Dilution Buffer
Magigen AapCas12b protein, derived from Alicyclobacillus acidiphilus, is an RNA-mediated endonuclease that binds to and cleaves specific sites of target DNA under the guidance of single-stranded RNA. While Cas12b recognizes and cleaves target double-stranded DNA under the guidance of guide RNA, its collateral cleavage activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA). CRISPR/Cas12b can be used to detect DNA templates and amplify signals by designing ssDNA probes labeled with fluorescent groups or other markers at both ends. Common detection methods include real-time fluorescence and colloidal gold chromatography. At present, this system has been widely used in gene editing of micro organisms, plants and animals, and has great practical application value in molecular diagnosis.
Storage conditions and Expiration
Storage conditions: -20℃, avoid light.
Expiration: 12 months. Avoid repeated freezing-thawing after opening.
Microplate Reader, like BioTek Synergy et al. fluorescence quantitative PCR instruments with FAM channel.
1. Preparation of reaction substrate
Reaction volume / final concentration
40 ~ 160nM
20 - 50 nM
100 - 500nM
up to 50 μL
2.2 Set up the program in advance and preheat while using microplate reader.
Filter Set: Green（Ex:485/20, Em: 528/20），Reaction temperature: 45 ~ 65℃, get the fluorescence values every 1 ~ 4 min, time: 30min ~ 1h.
3. 【Interpretation of the results】
CRISPR-Cas negative result and positive result
According to tested results below, using different concentrations of Cas12a protein to cut ssDNA, it can be found that a sufficient amount of Cas12a can cut DNA in about 12 minutes.
Magigen crispr cas12b protein is a qualified cas protein with good price.
The crispr cas12b price will be subject to change without prior notice due to currency fluctuation. All Pictures shown are for illustration purpose only and shall not form part of any offer, contract.
|CRISPR Cas12b protein | AapCas12b 1000p
|CRISPR Cas12a protein | LbaCas12a(Cpf1) 70p
|CRISPR Cas13a protein | LwCas13a 100p
|CRISPR Cas9 protein | spCas9 70p